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Anti-inflammatory and analgesic activity of ExCLzyme-EN®

S.L.Bodhankar, A.U.Burhan, V.M.Kale, S.Banerjee and S. Risbud

Bharati Vidyapeeth Deemed University, Poona College of Pharmacy, Pune 411 038.

Raj Biotech Pvt. Ltd., Pune 411038

Group Companies of Specialty Enzymes and Biochemicals Co., Chino, California 91710 and Advanced Biochemicals Ltd. Thane 400601.

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ExCLzyme-EN® (Group Companies of Specialty Enzymes and Biochemicals Co., Chino, California 91710 and Advanced Biochemicals Ltd. Thane 400601.) belongs to the class of “Proteolytic enzymes”. The enzymes inhibit release of pain inducing amines and facilitate drainage of exudates thereby promoting tissue repair process. They break down fibrin and inhibit the gathering of platelets around the healing site and protect tissue from damaging.  The objective was  to study anti-inflammatory and analgesic activity of ExCLzyme-EN®.

Carrageenan induced hind paw edema method of Winter et al, (1962) in rats was used.   ExCLzyme-EN® (100 mg/kg and 200 mg/kg, p.o.) showed a dose dependant inhibitory effect (49.73 % and 52.41 % inhibition).

Acetic acid induced writhing method of Taber et al, (1969) was used to study peripheral analgesic activity in mice.  ExCLzyme-EN® (40 mg/kg and 80 mg/kg, p.o.) showed a significant and dose dependant inhibitory effect (82.73 and 93.01% inhibition).

Carrageenan induced edema is biphasic mediated by release of histamine and 5-hydroxytryptamine (5-HT) during first hour, and kinine release up to 2 1/2 h. From 2 1/2 to 6 h the mediator appears to be prostaglandin, release of which is closely associated with migration of leukocytes into inflamed site.  ExCLzyme-EN® showed peak inhibitory effect at 3 h indicating inhibition of prostaglandin phase.

It is concluded that ExCLzyme-EN® posses significant anti-inflammatory and peripheral analgesic activity.

 
Table 1: Effect of Vehicle (Distilled water) / EXCLzyme™ EN (50 mg/kg, 100 mg/kg and 200 mg/kg) on carrageenan induced rat paw edema.
Table 2: Effect of EXCLzyme™-EN 100mg/kg and 200 mg/kg on Freund's adjuvant induced arthritis in rats.
Table 3: Effect of EXCLzyme™-EN on Acetic acid induced writhing in mice.
 
Table 1: Effect of Vehicle (Distilled water), EXCLzyme™ EN, Wobenzyme (both same dose; 200 mg/kg) on carrageenan induced rat paw edema.
Table 2: Effect of EXCLzyme™ EN and Wobenzyme (both same dose; 200 mg/kg) on Freund's adjuvant induced arthritis in rats.
Table 3: Effect of EXCLzyme™-EN on Acetic acid induced writhing in mice.

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